Brand | Abnova |
Product type | Antibody pair |
Reactivity | Human |
Applications | PLA-Ce |
Brand: | Abnova |
Reference: | DI0123 |
Product name: | HNF4A & CTNNB1 Protein Protein Interaction Antibody Pair |
Product description: | This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the HNF4A protein, and the other against the CTNNB1 protein for use in in situ Proximity Ligation Assay. See Publication Reference below. |
Gene id: | 3172|1499 |
Gene name: | HNF4A |
Gene alias: | FLJ39654|HNF4|HNF4a7|HNF4a8|HNF4a9|MODY|MODY1|NR2A1|NR2A21|TCF|TCF14 |
Gene description: | hepatocyte nuclear factor 4, alpha |
Supplied product: | Antibody pair set content: 1. HNF4A rabbit purified polyclonal antibody (20 ug) 2. CTNNB1 mouse monoclonal antibody (40 ug) *Reagents are sufficient for at least 30-50 assays using recommended protocols. |
Storage instruction: | Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use. |
Quality control testing: | Protein protein interaction immunofluorescence result. |
Quality control testing picture: | |
Quality control testing picture note: | Representative image of Proximity Ligation Assay of protein-protein interactions between HNF4A and CTNNB1. HeLa cells were stained with anti-HNF4A rabbit purified polyclonal antibody 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University. |
Product type: | Antibody pair |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: | |
Application image note: | Representative image of Proximity Ligation Assay of protein-protein interactions between HNF4A and CTNNB1. A-549 cells were stained with anti-HNF4A rabbit purified polyclonal antibody 1:100 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). |
Applications: | PLA-Ce |
Shipping condition: | Dry Ice |