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Data sheet of ASAH1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
| Brand | Abnova |
| Product type | Lysate |
| Host species | Human |
| Applications | WB,IP |
More info about ASAH1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
| Brand: | Abnova |
| Reference: | L146T6 |
| Product name: | ASAH1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured) |
| Gene id: | 427 |
| Gene name: | ASAH1 |
| Gene alias: | AC|ASAH|FLJ21558|FLJ22079|PHP|PHP32 |
| Gene description: | N-acylsphingosine amidohydrolase (acid ceramidase) 1 |
| Genbank accession: | NM_177924 |
| Protein accession: | NP_808592 |
| Concentration: | 2 mg/ml |
| Plasmid: | pCMV-ASAH1 full length |
| Lysis buffer: | Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Host cell: | HEK293 |
| Recommend dilutions: | Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
| Storage buffer: | In modified RIPA Lysis Buffer. |
| Storage instruction: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Transient overexpression cell lysate was tested with Anti-ASAH1 antibody (H00000427-M01 ) by Western Blots. |
| Quality control testing picture: |  |
| Quality control testing picture note: | ASAH1 transfected lysate |
| Product type: | Lysate |
| Host species: | Human |
| Antigen species / target species: | Human |
| Applications: | WB,IP |
| Shipping condition: | Dry Ice |