LIPA HEK293 Cell Transient Overexpression Lysate(Non-Denatured) View larger

LIPA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

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Data sheet of LIPA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

BrandAbnova
Product typeLysate
Host speciesHuman
ApplicationsWB,IP

More info about LIPA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

Brand: Abnova
Reference: L136T6
Product name: LIPA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
Gene id: 3988
Gene name: LIPA
Gene alias: CESD|LAL
Gene description: lipase A, lysosomal acid, cholesterol esterase
Genbank accession: BC012287
Protein accession: AAH12287
Concentration: 2 mg/ml
Plasmid: pCMV-LIPA full length
Lysis buffer: Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Host cell: HEK293
Recommend dilutions: Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage buffer: In modified RIPA Lysis Buffer.
Storage instruction: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Quality control testing: Transient overexpression cell lysate was tested with Anti-LIPA antibody (H00003988-M01) by Western Blots.
Quality control testing picture: qc_test-L136T6-1.jpg
Quality control testing picture note: LIPA transfected lysate
Product type: Lysate
Host species: Human
Antigen species / target species: Human
Applications: WB,IP
Shipping condition: Dry Ice

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