GBA HEK293 Cell Transient Overexpression Lysate(Non-Denatured) View larger

GBA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

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Data sheet of GBA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

BrandAbnova
Product typeLysate
Host speciesHuman
ApplicationsWB,IP

More info about GBA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

Brand: Abnova
Reference: L100T6
Product name: GBA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
Gene id: 2629
Gene name: GBA
Gene alias: GBA1|GCB|GLUC
Gene description: glucosidase, beta; acid (includes glucosylceramidase)
Genbank accession: NM_000157
Protein accession: NP_000148
Concentration: 2 mg/ml
Plasmid: pCMV-GBA full length
Lysis buffer: Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Host cell: HEK293
Recommend dilutions: Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage buffer: In modified RIPA Lysis Buffer.
Storage instruction: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Quality control testing: Transient overexpression cell lysate was tested with Anti-GBA antibody (H00002629-M01) by Western Blots.
Quality control testing picture: qc_test-L100T6-1.jpg
Quality control testing picture note: GBA transfected lysate
Product type: Lysate
Host species: Human
Antigen species / target species: Human
Applications: WB,IP
Shipping condition: Dry Ice

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