Brand: | Abnova |
Reference: | L087T6 |
Product name: | EVL HEK293 Cell Transient Overexpression Lysate(Non-Denatured) |
Gene id: | 51466 |
Gene name: | EVL |
Gene alias: | RNB6 |
Gene description: | Enah/Vasp-like |
Genbank accession: | NM_016337 |
Protein accession: | NP_057421 |
Concentration: | 2 mg/ml |
Plasmid: | pCMV-EVL full length |
Lysis buffer: | Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
Host cell: | HEK293 |
Recommend dilutions: | Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Storage buffer: | In modified RIPA Lysis Buffer. |
Storage instruction: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Transient overexpression cell lysate was tested with Anti-EVL antibody (H00051466-M01) by Western Blots. |
Quality control testing picture: | |
Quality control testing picture note: | EVL transfected lysate |
Product type: | Lysate |
Host species: | Human |
Antigen species / target species: | Human |
Applications: | WB,IP |
Shipping condition: | Dry Ice |