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Brand | Abnova |
Product type | Lysate |
Host species | Human |
Applications | WB,IP |
Brand: | Abnova |
Reference: | L086T6 |
Product name: | ETV4 HEK293 Cell Transient Overexpression Lysate(Non-Denatured) |
Gene id: | 2118 |
Gene name: | ETV4 |
Gene alias: | E1A-F|E1AF|PEA3|PEAS3 |
Gene description: | ets variant 4 |
Genbank accession: | BC007242 |
Protein accession: | AAH07242 |
Concentration: | 2 mg/ml |
Plasmid: | pCMV-ETV4 full length |
Lysis buffer: | Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
Host cell: | HEK293 |
Recommend dilutions: | Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Storage buffer: | In modified RIPA Lysis Buffer. |
Storage instruction: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Transient overexpression cell lysate was tested with Anti-ETV4 antibody (H00002118-M01) by Western Blots. |
Quality control testing picture: | |
Quality control testing picture note: | ETV4 transfected lysate |
Product type: | Lysate |
Host species: | Human |
Antigen species / target species: | Human |
Applications: | WB,IP |
Shipping condition: | Dry Ice |