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Brand | Abnova |
Product type | Lysate |
Host species | Human |
Applications | WB,IP |
Brand: | Abnova |
Reference: | L047T6 |
Product name: | CRADD HEK293 Cell Transient Overexpression Lysate(Non-Denatured) |
Gene id: | 8738 |
Gene name: | CRADD |
Gene alias: | MGC9163|RAIDD |
Gene description: | CASP2 and RIPK1 domain containing adaptor with death domain |
Genbank accession: | BC037905 |
Protein accession: | AAH37905 |
Concentration: | 2 mg/ml |
Plasmid: | pCMV-CRADD full length |
Lysis buffer: | Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
Host cell: | HEK293 |
Recommend dilutions: | Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Storage buffer: | In modified RIPA Lysis Buffer. |
Storage instruction: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Transient overexpression cell lysate was tested with Anti-CRADD antibody (H00008738-M01) by Western Blots. |
Quality control testing picture: | |
Quality control testing picture note: | CRADD transfected lysate |
Product type: | Lysate |
Host species: | Human |
Antigen species / target species: | Human |
Applications: | WB,IP |
Shipping condition: | Dry Ice |