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Data sheet of MCF-7(human breast adenocarcinoma) whole cell lysate (denatured)
| Brand | Abnova |
| Product type | Lysate |
| Host species | Human |
| Applications | WB |
More info about MCF-7(human breast adenocarcinoma) whole cell lysate (denatured)
| Brand: | Abnova |
| Reference: | L046V1 |
| Product name: | MCF-7(human breast adenocarcinoma) whole cell lysate (denatured) |
| Product description: | Whole cell lysate (denatured) |
| Lysis buffer: | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Tissue: | Mammary Gland, Breast |
| Preparation method: | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly. |
| Recommend dilutions: | The lysate is ready to load on SDS-PAGE for Western blotting. |
| Storage buffer: | In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue). |
| Storage instruction: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | 12.5% SDS-PAGE Stained with Coomassie Blue. |
| Quality control testing picture: |  |
| Product type: | Lysate |
| Host species: | Human |
| Antigen species / target species: | Human |
| Applications: | WB |
| Shipping condition: | Dry Ice |