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Data sheet of hES-TW1(human embryonic stem cell) whole cell lysate (non-denatured)
| Brand | Abnova |
| Product type | Lysate |
| Host species | Human |
| Applications | WB,IP |
More info about hES-TW1(human embryonic stem cell) whole cell lysate (non-denatured)
| Brand: | Abnova |
| Reference: | L043V2 |
| Product name: | hES-TW1(human embryonic stem cell) whole cell lysate (non-denatured) |
| Product description: | Whole cell lysate (non-denatured) |
| Lysis buffer: | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Tissue: | Embryonic |
| Preparation method: | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). |
| Recommend dilutions: | Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
| Storage buffer: | In modified RIPA Lysis Buffer. |
| Storage instruction: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | 12.5% SDS-PAGE Stained with Coomassie Blue. |
| Quality control testing picture: |  |
| Product type: | Lysate |
| Host species: | Human |
| Antigen species / target species: | Human |
| Applications: | WB,IP |
| Shipping condition: | Dry Ice |
| Publications: | Combinatorial Patterning of Chromatin Regulators Uncovered by Genome-wide Location Analysis in Human Cells.Ram O, Goren A, Amit I, Shoresh N, Yosef N, Ernst J, Kellis M, Gymrek M, Issner R, Coyne M, Durham T, Zhang X, Donaghey J, Epstein CB, Regev A, Bernstein BE. Cell. 2011 Dec 23;147(7):1628-39. |