Rat heart tissue lysate (non-denatured) View larger

Rat heart tissue lysate (non-denatured)

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172,00 € tax excl.

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Data sheet of Rat heart tissue lysate (non-denatured)

BrandAbnova
Product typeLysate
Host speciesRat
ApplicationsWB,IP

More info about Rat heart tissue lysate (non-denatured)

Brand: Abnova
Reference: L036W2
Product name: Rat heart tissue lysate (non-denatured)
Product description: Whole tissue lysate (non-denatured)
Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Tissue: Heart
Preparation method: Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Recommend dilutions: Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage buffer: In modified RIPA Lysis Buffer.
Storage instruction: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Quality control testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Quality control testing picture: qc_test-L036W2-1.jpg
Product type: Lysate
Host species: Rat
Antigen species / target species: Rat
Applications: WB,IP
Shipping condition: Dry Ice

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