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Mouse brain tissue lysate (denatured)

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Data sheet of Mouse brain tissue lysate (denatured)

BrandAbnova
Product typeLysate
Host speciesMouse
ApplicationsWB

More info about Mouse brain tissue lysate (denatured)

Brand: Abnova
Reference: L035W1
Product name: Mouse brain tissue lysate (denatured)
Product description: Whole tissue lysate (denatured)
Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Tissue: Brain
Preparation method: Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 3.75 mg/ml, and then mixed with 5X Sample Buffer to become final 3 mg/ml in 1X Sample Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Recommend dilutions: The lysate is ready to load on SDS-PAGE for Western blotting.
Storage buffer: In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Storage instruction: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Quality control testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Quality control testing picture: qc_test-L035W1-1.jpg
Product type: Lysate
Host species: Mouse
Antigen species / target species: Mouse
Applications: WB
Shipping condition: Dry Ice

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