A-549 (human lung carcinoma) nuclear extract lysate (non-denatured) View larger

A-549 (human lung carcinoma) nuclear extract lysate (non-denatured)

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Data sheet of A-549 (human lung carcinoma) nuclear extract lysate (non-denatured)

BrandAbnova
Product typeLysate
Host speciesHuman
ApplicationsWB,IP

More info about A-549 (human lung carcinoma) nuclear extract lysate (non-denatured)

Brand: Abnova
Reference: L025V4
Product name: A-549 (human lung carcinoma) nuclear extract lysate (non-denatured)
Product description: Nuclear extract cell lysate (non-denatured)
Lysis buffer: Buffer A: 10mM HEPES pH 7.9, 1.5mM MgCl2, 10mM KCl, 0.5 mM DTT.
Buffer C: 20mM HEPES pH 7.9, 25%(v/v) Glycerol , 0.42M NaCl , 1.5mM MgCl2, 0.2 mM EDTA, 0.5 mM DTT & 0.5 mM PMSF.
Buffer D : 20mM HEPES pH 7.9, 20%(v/v) glycerol, 50mM KCl, 0.2 mM EDTA, 0.5 mM DTT & 0.5 mM PMSF.
Tissue: Lung
Preparation method: Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A, and then centrifugated at 25,000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffer C, and then the suspensions were centrifuged to collect nuclear extract. The supernatant was dialyzed against Buffer D. The dialysate was then centrifuged, divided into aliquots, and stored at -80°C. The protein concentration was determined by the method of Bradford (Bio-Rad protein assay, microplate standard assay). The lysate was adjusted to 2 mg/ml.
Recommend dilutions: Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage buffer: In Buffer D.
Storage instruction: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Quality control testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Quality control testing picture: qc_test-L025V4-1.jpg
Product type: Lysate
Host species: Human
Antigen species / target species: Human
Applications: WB,IP
Shipping condition: Dry Ice

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