Raw 264.7 (mouse macrophage, Abelson murine leukemia virus transformed) whole cell lysate (denatured) View larger

Raw 264.7 (mouse macrophage, Abelson murine leukemia virus transformed) whole cell lysate (denatured)

New product

172,00 € tax excl.

Customer ratings and reviews

Nobody has posted a review yet
in this language

Data sheet of Raw 264.7 (mouse macrophage, Abelson murine leukemia virus transformed) whole cell lysate (denatured)

BrandAbnova
Product typeLysate
Host speciesMouse
ApplicationsWB

More info about Raw 264.7 (mouse macrophage, Abelson murine leukemia virus transformed) whole cell lysate (denatured)

Brand: Abnova
Reference: L024V1
Product name: Raw 264.7 (mouse macrophage, Abelson murine leukemia virus transformed) whole cell lysate (denatured)
Product description: Whole cell lysate (denatured)
Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Tissue: Macrophage
Preparation method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Recommend dilutions: The lysate is ready to load on SDS-PAGE for Western blotting.
Storage buffer: In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Storage instruction: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Quality control testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Quality control testing picture: qc_test-L024V1-1.jpg
Product type: Lysate
Host species: Mouse
Antigen species / target species: Mouse
Applications: WB
Shipping condition: Dry Ice

Reviews

Buy Raw 264.7 (mouse macrophage, Abelson murine leukemia virus transformed) whole cell lysate (denatured) now

Add to cart