New product
Availability date:
Brand | Abnova |
Product type | Lysate |
Host species | Human |
Applications | WB,IP |
Brand: | Abnova |
Reference: | L021V2 |
Product name: | MES-SA/Dx5 (human uterine sarcoma, MDR variant) whole cell lysate (non-denatured) |
Product description: | Whole cell lysate (non-denatured) |
Lysis buffer: | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
Tissue: | Uterus |
Preparation method: | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). |
Recommend dilutions: | Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Storage buffer: | In modified RIPA Lysis Buffer. |
Storage instruction: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | 12.5% SDS-PAGE Stained with Coomassie Blue. |
Quality control testing picture: | |
Product type: | Lysate |
Host species: | Human |
Antigen species / target species: | Human |
Applications: | WB,IP |
Shipping condition: | Dry Ice |