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Data sheet of THP-1 (human acute monocytic leukemia) whole cell lysate (non-denatured)
| Brand | Abnova |
| Product type | Lysate |
| Host species | Human |
| Applications | WB,IP |
More info about THP-1 (human acute monocytic leukemia) whole cell lysate (non-denatured)
| Brand: | Abnova |
| Reference: | L007V2 |
| Product name: | THP-1 (human acute monocytic leukemia) whole cell lysate (non-denatured) |
| Product description: | Whole cell lysate (non-denatured) |
| Lysis buffer: | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Tissue: | Peripheral Blood |
| Preparation method: | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). |
| Recommend dilutions: | Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
| Storage buffer: | In modified RIPA Lysis Buffer. |
| Storage instruction: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | 12.5% SDS-PAGE Stained with Coomassie Blue. |
| Quality control testing picture: |  |
| Product type: | Lysate |
| Host species: | Human |
| Antigen species / target species: | Human |
| Applications: | WB,IP |
| Shipping condition: | Dry Ice |
| Publications: | Reciprocal coupling of coagulation and innate immunity via neutrophil serine proteases.Massberg S, Grahl L, von Bruehl ML, Manukyan D, Pfeiler S, Goosmann C, Brinkmann V, Lorenz M, Bidzhekov K, Khandagale AB, Konrad I, Kennerknecht E, Reges K, Holdenrieder S, Braun S, Reinhardt C, Spannagl M, Preissner KT, Engelmann B. Nat Med. 2010 Aug;16(8):887-96. Epub 2010 Aug 1. |