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Histone H4 (K5ac) polyclonal antibody

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Data sheet of Histone H4 (K5ac) polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman,Mouse
Host speciesRabbit
ApplicationsWB,ELISA,ChIP,ChIP-Seq,Dot,IF

More info about Histone H4 (K5ac) polyclonal antibody

Brand: Abnova
Reference: PAB31333
Product name: Histone H4 (K5ac) polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of Histone H4 (K5ac).
Gene id: 8359
Gene name: HIST1H4A
Gene alias: H4/a|H4FA
Gene description: histone cluster 1, H4a
Immunogen: A synthetic peptide (conjugated with KLH) corresponding to Histone H4, acetylated at lysine 5.
Protein accession: P62805
Form: Liquid
Recommend dilutions: ELISA (1:100-500)
Western Blot (1:500)
ChIP (1-2 ug/IP)
Dot Blot (1:5000)
Immunofluorescence (1:500)
The optimal working dilution should be determined by the end user.
Storage buffer: In PBS (0.05% sodium azide, 0.05% proclin 300).
Storage instruction: Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Tag: KLH
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human,Mouse
Application image: PAB31333-20-1-1.jpg
Application image note: ChIP assays were performed using human HeLa cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Applications: WB,ELISA,ChIP,ChIP-Seq,Dot,IF
Shipping condition: Dry Ice

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