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Data sheet of Histone H4 (K5,8,12ac) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Rabbit |
| Applications | WB,ELISA,ChIP,ChIP-Seq,Dot,IF |
More info about Histone H4 (K5,8,12ac) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB31332 |
| Product name: | Histone H4 (K5,8,12ac) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Histone H4 (K5,8,12ac). |
| Gene id: | 8359 |
| Gene name: | HIST1H4A |
| Gene alias: | H4/a|H4FA |
| Gene description: | histone cluster 1, H4a |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to Histone H4, acetylated at lysines 5, 8, and 12. |
| Protein accession: | P62805 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:1000) Western Blot (1:1000) ChIP (0.5-1 ug/IP) Dot Blot (1:20000) Immunofluorescence (1:500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.05% sodium azide, 0.05% proclin 300). |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Tag: | KLH |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human,Mouse |
| Application image: |  |
| Application image note: | ChIP assays were performed using human K562 cells. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). |
| Applications: | WB,ELISA,ChIP,ChIP-Seq,Dot,IF |
| Shipping condition: | Dry Ice |