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Data sheet of Histone H3 (R17me2(asym)K18ac)) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Rabbit |
| Applications | WB,ELISA,ChIP,ChIP-Seq,Dot,IF |
More info about Histone H3 (R17me2(asym)K18ac)) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB31326 |
| Product name: | Histone H3 (R17me2(asym)K18ac)) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (R17me2(asym)K18ac)). |
| Gene id: | 8350 |
| Gene name: | HIST1H3A |
| Gene alias: | H3/A|H3FA |
| Gene description: | histone cluster 1, H3a |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to Histone H3, asymmetrically dimethylated at arginine 17 and acetylated at lysine 18. |
| Protein accession: | P68431 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:1000) Western Blot (1:1000) ChIP (1 ug/IP) Dot Blot (1:20000) Immunofluorescence (1:500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.05% sodium azide, 0.05% proclin 300). |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Tag: | KLH |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human,Mouse |
| Application image: |  |
| Application image note: | ChIP assays were performed using human HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). |
| Applications: | WB,ELISA,ChIP,ChIP-Seq,Dot,IF |
| Shipping condition: | Dry Ice |