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Histone H3 (K4me1) polyclonal antibody

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Data sheet of Histone H3 (K4me1) polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman,Mouse
Host speciesRabbit
ApplicationsWB,ELISA,ChIP,ChIP-Seq,Dot,IF

More info about Histone H3 (K4me1) polyclonal antibody

Brand: Abnova
Reference: PAB31324
Product name: Histone H3 (K4me1) polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K4me1).
Gene id: 8350
Gene name: HIST1H3A
Gene alias: H3/A|H3FA
Gene description: histone cluster 1, H3a
Immunogen: A synthetic peptide (conjugated with KLH) corresponding to Histone H3, monomethylated at lysine 4.
Protein accession: P68431
Form: Liquid
Recommend dilutions: ELISA (1:400)
Western Blot (1:500)
ChIP (0.5-1 ug/IP)
Dot Blot (1:5000)
Immunofluorescence (1:200)
The optimal working dilution should be determined by the end user.
Storage buffer: In PBS (0.05% sodium azide, 0.05% proclin 300).
Storage instruction: Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Tag: KLH
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human,Mouse
Application image: PAB31324-20-9-1.jpg
Application image note: ChIP assays were performed using human K562 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for a region surrounding the ACTB and GAS2L1 gene, respectively, used as positive controls, and for the promoters of the GAPDH and EIF4A2 genes, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Applications: WB,ELISA,ChIP,ChIP-Seq,Dot,IF
Shipping condition: Dry Ice
Publications: Chromatin immunoprecipitation from fixed clinical tissues reveals tumor-specific enhancer profiles.Cejas P, Li L, ONeill NK, Duarte M, Rao P, Bowden M, Zhou CW, Mendiola M, Burgos E, Feliu J, Moreno-Rubio J, Guadalajara H, Moreno V, Garcia-Olmo D, Bellmunt J, Mullane S, Hirsch M, Sweeney CJ, Richardson A, Liu XS, Brown M, Shivdasani RA, Long HW.
Nat Med. 2016 Jun;22(6):685-91.

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