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Data sheet of Histone H3 (K36me3) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Rabbit |
| Applications | ELISA,ChIP,ChIP-Seq,Dot,WB-Ce |
More info about Histone H3 (K36me3) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB31321 |
| Product name: | Histone H3 (K36me3) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K36me3). |
| Gene id: | 8350 |
| Gene name: | HIST1H3A |
| Gene alias: | H3/A|H3FA |
| Gene description: | histone cluster 1, H3a |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to Histone H3, trimethylated at lysine 36. |
| Protein accession: | P68431 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:100-500) Western Blot (1:1000) ChIP (5-10 ul/CHIP) Dot Blot (1:100000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In whole antiserum (0.05% sodium azide). |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Tag: | KLH |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human,Mouse |
| Application image: |  |
| Application image note: | ChIP assays were performed using human osteosarcoma (U2OS) cells. A titration of the antibody consisting of 2, 5, 10 and 15 ul per ChIP experiment was analysed. IgG (5 ug/IP) was used as a negative IP control. Quantitative PCR was performed using primer sets for the housekeeping gene GAPDH and for myogenic differentiation gene (MYOD). The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis. |
| Applications: | ELISA,ChIP,ChIP-Seq,Dot,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | The transcriptional and epigenomic foundations of ground state pluripotency.Marks H, Kalkan T, Menafra R, Denissov S, Jones K, Hofemeister H, Nichols J, Kranz A, Stewart AF, Smith A, Stunnenberg HG. Cell. 2012 Apr 27;149(3):590-604. |