Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Human |
Host species | Rabbit |
Applications | WB,ELISA,ChIP,ChIP-Seq,Dot,WB-Ce |
Brand: | Abnova |
Reference: | PAB31319 |
Product name: | Histone H3 (K27me3)(phospho S28) polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K27me3)(phospho S28). |
Gene id: | 8350 |
Gene name: | HIST1H3A |
Gene alias: | H3/A|H3FA |
Gene description: | histone cluster 1, H3a |
Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to Histone H3, trimethylated at lysine 27 and phosphorylated at serine 28. |
Protein accession: | P68431 |
Form: | Liquid |
Recommend dilutions: | ELISA (1:200) Western Blot (1:1000) ChIP (1-2 ug/IP) Dot Blot (1:20000) The optimal working dilution should be determined by the end user. |
Storage buffer: | In PBS (0.05% sodium azide, 0.05% proclin 300). |
Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Tag: | KLH |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: | |
Application image note: | ChIP assays were performed using human HeLa cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active gene GAPDH as a negative control, and for the coding regions of the inactive genes MYT1 and TSH2B as positive controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me3S28p is preferably present at inactive genes. |
Applications: | WB,ELISA,ChIP,ChIP-Seq,Dot,WB-Ce |
Shipping condition: | Dry Ice |