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Histone H3 (K27me3)(phospho S28) polyclonal antibody

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Data sheet of Histone H3 (K27me3)(phospho S28) polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
Host speciesRabbit
ApplicationsWB,ELISA,ChIP,ChIP-Seq,Dot,WB-Ce

More info about Histone H3 (K27me3)(phospho S28) polyclonal antibody

Brand: Abnova
Reference: PAB31319
Product name: Histone H3 (K27me3)(phospho S28) polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K27me3)(phospho S28).
Gene id: 8350
Gene name: HIST1H3A
Gene alias: H3/A|H3FA
Gene description: histone cluster 1, H3a
Immunogen: A synthetic peptide (conjugated with KLH) corresponding to Histone H3, trimethylated at lysine 27 and phosphorylated at serine 28.
Protein accession: P68431
Form: Liquid
Recommend dilutions: ELISA (1:200)
Western Blot (1:1000)
ChIP (1-2 ug/IP)
Dot Blot (1:20000)
The optimal working dilution should be determined by the end user.
Storage buffer: In PBS (0.05% sodium azide, 0.05% proclin 300).
Storage instruction: Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Tag: KLH
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human
Application image: PAB31319-20-1-1.jpg
Application image note: ChIP assays were performed using human HeLa cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active gene GAPDH as a negative control, and for the coding regions of the inactive genes MYT1 and TSH2B as positive controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me3S28p is preferably present at inactive genes.
Applications: WB,ELISA,ChIP,ChIP-Seq,Dot,WB-Ce
Shipping condition: Dry Ice

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