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Data sheet of Histone H3 (K27me3) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Fruit fly,Human,Mouse,Nematoda,Arabidopsis,Maize,Solanum lycopersicum,Populus |
| Host species | Rabbit |
| Applications | WB,ELISA,ChIP,ChIP-Seq,Dot,IF |
More info about Histone H3 (K27me3) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB31318 |
| Product name: | Histone H3 (K27me3) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K27me3). |
| Gene id: | 8350 |
| Gene name: | HIST1H3A |
| Gene alias: | H3/A|H3FA |
| Gene description: | histone cluster 1, H3a |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to Histone H3, trimethylated at lysine 27. |
| Protein accession: | P68431 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:1000) Western Blot (1:1000) ChIP (1 ug/IP) Dot Blot/Peptide array (1:20000) Immunofluorescence (1:500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.05% sodium azide, 0.05% proclin 300). |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Tag: | KLH |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Fruit fly,Human,Mouse,Nematoda,Arabidopsis,Maize,Solanum lycopersicum,Populus |
| Application image: |  |
| Application image note: | ChIP assays were performed using human K562 cells. A titration consisting of 0.1, 0.5, and 1 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as negative controls, and TSH2B and MYT1, used as positive controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). |
| Applications: | WB,ELISA,ChIP,ChIP-Seq,Dot,IF |
| Shipping condition: | Dry Ice |
| Publications: | DNA methylation heterogeneity defines a disease spectrum in Ewing sarcoma.Sheffield NC, Pierron G, Klughammer J, Datlinger P, Schonegger A, Schuster M, Hadler J, Surdez D, Guillemot D, Lapouble E, Freneaux P, Champigneulle J, Bouvier R, Walder D, Ambros IM, Hutter C, Sorz E, Amaral AT, de Alava E, Schallmoser K, Strunk D, Rinner B, Liegl-Atzwanger B, Huppertz B, Leithner A, de Pinieux G, Terrier P, Laurence V, Michon J, Ladenstein R, Holter W, Windhager R, Dirksen U, Ambros PF, Delattre O, Kovar H, Bock C, Tomazou EM. Nat Med. 2017 Mar;23(3):386-395. |