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Histone H2B (K15ac) polyclonal antibody

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Data sheet of Histone H2B (K15ac) polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
Host speciesRabbit
ApplicationsWB,ELISA,ChIP,ChIP-Seq,Dot,IF

More info about Histone H2B (K15ac) polyclonal antibody

Brand: Abnova
Reference: PAB31313
Product name: Histone H2B (K15ac) polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of Histone H2B (K15ac).
Gene id: 8347
Gene name: HIST1H2BC
Gene alias: H2B.1|H2B/l|H2BFL|MGC104246|dJ221C16.3
Gene description: histone cluster 1, H2bc
Immunogen: A synthetic peptide (conjugated with KLH) corresponding to Histone H2B, acetylated at lysine 15.
Protein accession: P62807
Form: Liquid
Recommend dilutions: ELISA (1:1000)
Western Blot (1:500)
ChIP (2 ug/ChIP)
Dot Blot (1:20000)
Immunofluorescence (1:500)
The optimal working dilution should be determined by the end user.
Storage buffer: In PBS (0.05% sodium azide, 0.05% proclin 300).
Storage instruction: Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Tag: KLH
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human
Application image: PAB31313-20-1-1.jpg
Application image note: ChIP assays were performed using human HeLa cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Applications: WB,ELISA,ChIP,ChIP-Seq,Dot,IF
Shipping condition: Dry Ice

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