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Histone H2AZ polyclonal antibody

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Data sheet of Histone H2AZ polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
Host speciesRabbit
ApplicationsELISA,ChIP,ChIP-Seq,Dot,IF

More info about Histone H2AZ polyclonal antibody

Brand: Abnova
Reference: PAB31309
Product name: Histone H2AZ polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of Histone H2AZ.
Gene id: 3015
Gene name: H2AFZ
Gene alias: H2A.z|H2A/z|H2AZ|MGC117173
Gene description: H2A histone family, member Z
Immunogen: A synthetic peptide (conjugated with KLH) corresponding to Histone HAZ, acetylated at lysines 4, 7 and 11.
Protein accession: P0C0S5
Form: Liquid
Recommend dilutions: ELISA (1:200)
ChIP (1 ug/ChIP)
Dot Blot (1:20000)
Immunofluorescence (1:500)
The optimal working dilution should be determined by the end user.
Storage buffer: In PBS (0.05% sodium azide, 0.05% proclin 300).
Storage instruction: Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Tag: KLH
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human
Application image: PAB31309-20-1-1.jpg
Application image note: ChIP assays were performed using human HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. QPCR was performed using primers specific for the promoters of the ACTB and EIF4A2 genes, used as positive control targets and for the coding region of the MYT1 gene, used as a negative control target. The figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA). These results confirm the observation that acetylation of Histone H2AZ is present at active promoters.
Applications: ELISA,ChIP,ChIP-Seq,Dot,IF
Shipping condition: Dry Ice
Publications: CpG signalling, H2A.Z/H3 acetylation and microRNA-mediated deferred self-attenuation orchestrate foetal NOS3 expression.Postberg J, Kanders M, Forcob S, Willems R, Orth V, Hensel KO, Weil PP, Wirth S, Jenke AC.
Clin Epigenetics. 2015 Feb 8;7:9.

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