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Data sheet of Histone H3 (phospho S10) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,ChIP,Dot,WB-Ce,IF |
More info about Histone H3 (phospho S10) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB31273 |
| Product name: | Histone H3 (phospho S10) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (phospho S10). |
| Gene id: | 8350 |
| Gene name: | HIST1H3A |
| Gene alias: | H3/A|H3FA |
| Gene description: | histone cluster 1, H3a |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to human histone H3, phosphorylated at serine 10. |
| Protein accession: | P68431 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:100) Western Blot (1:1000) ChIP (2 ug/CHIP) Dot Blot (1:20000) Immunofluorescence (1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.05% sodium azide, 0.05% proclin 300). |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Tag: | KLH |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Cross reactivity test with the Histone H3 (phospho S10) antibody. Dot Blot analysis was performed with peptides containing other modifications of histone H3 or the unmodified H3 (phospho S10) sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20000. The figure shows a high specificity of the antibody for the modification of interest. Note that the antibody does not recognize the Histone H3 (phospho S10) modification if the neighboring K9 is acetylated or trimethylated. |
| Applications: | ELISA,ChIP,Dot,WB-Ce,IF |
| Shipping condition: | Dry Ice |
| Publications: | ERK-Induced Activation of TCF Family of SRF Cofactors Initiates a Chromatin Modification Cascade Associated with Transcription.Esnault C. et al. Mol Cell.2017 Mar 16;65(6):1081-1095.e5. |