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Histone H3 (K9me1) polyclonal antibody

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Data sheet of Histone H3 (K9me1) polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
Host speciesRabbit
ApplicationsELISA,ChIP,Dot,WB-Ce

More info about Histone H3 (K9me1) polyclonal antibody

Brand: Abnova
Reference: PAB31270
Product name: Histone H3 (K9me1) polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K9me1).
Gene id: 8350
Gene name: HIST1H3A
Gene alias: H3/A|H3FA
Gene description: histone cluster 1, H3a
Immunogen: A synthetic peptide (conjugated with KLH) corresponding to Histone H3, monomethylated at lysine 9.
Protein accession: P68431
Form: Liquid
Recommend dilutions: ELISA (1:500-1000)
Western Blot (1:1000)
ChIP (1 ug/CHIP)
Dot Blot (1:20000)
The optimal working dilution should be determined by the end user.
Storage buffer: In PBS (0.05% sodium azide, 0.05% proclin 300).
Storage instruction: Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Tag: KLH
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human
Application image: PAB31270-43-outsr-1.jpg
Application image note: Cross reactivity test of the Histone H3 (K9me1) antibody.
Dot Blot analysis was performed with peptides containing other modifications and the unmodified sequence of histone H3. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20000. The figure shows a high specificity of the antibody for the modification of interest.
Applications: ELISA,ChIP,Dot,WB-Ce
Shipping condition: Dry Ice
Publications: The histone demethylase enzyme KDM3A is a key estrogen receptor regulator in breast cancer.Wade MA, Jones D, Wilson L, Stockley J, Coffey K, Robson CN, Gaughan L.
Nucleic Acids Res.2015 Jan;43(1):196-207.

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