Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Human |
Host species | Rabbit |
Applications | ELISA,ChIP,Dot,WB-Ce |
Brand: | Abnova |
Reference: | PAB31270 |
Product name: | Histone H3 (K9me1) polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K9me1). |
Gene id: | 8350 |
Gene name: | HIST1H3A |
Gene alias: | H3/A|H3FA |
Gene description: | histone cluster 1, H3a |
Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to Histone H3, monomethylated at lysine 9. |
Protein accession: | P68431 |
Form: | Liquid |
Recommend dilutions: | ELISA (1:500-1000) Western Blot (1:1000) ChIP (1 ug/CHIP) Dot Blot (1:20000) The optimal working dilution should be determined by the end user. |
Storage buffer: | In PBS (0.05% sodium azide, 0.05% proclin 300). |
Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Tag: | KLH |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: | |
Application image note: | Cross reactivity test of the Histone H3 (K9me1) antibody. Dot Blot analysis was performed with peptides containing other modifications and the unmodified sequence of histone H3. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20000. The figure shows a high specificity of the antibody for the modification of interest. |
Applications: | ELISA,ChIP,Dot,WB-Ce |
Shipping condition: | Dry Ice |
Publications: | The histone demethylase enzyme KDM3A is a key estrogen receptor regulator in breast cancer.Wade MA, Jones D, Wilson L, Stockley J, Coffey K, Robson CN, Gaughan L. Nucleic Acids Res.2015 Jan;43(1):196-207. |