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Data sheet of Histone H3 (K4me2) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Rabbit |
| Applications | ELISA,ChIP,Dot,WB-Ce |
More info about Histone H3 (K4me2) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB31262 |
| Product name: | Histone H3 (K4me2) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K4me2). |
| Gene id: | 8350 |
| Gene name: | HIST1H3A |
| Gene alias: | H3/A|H3FA |
| Gene description: | histone cluster 1, H3a |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to Histone H3, dimethylated at lysine 4. |
| Protein accession: | P68431 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:300) Western Blot (1:750) ChIP (10 ul/CHIP) Dot Blot (1:20000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.05% sodium azide). |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Tag: | KLH |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human,Mouse |
| Application image: |  |
| Application image note: | ChIP assays were performed using human osteosarcoma (U2OS) cells. A titration of the antibody consisting of 2, 5, 10 or 15 ul per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for the promoter of the ALDOA gene and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that dimethylation of K4 at histone H3 is more present at active genes than at silent genes. |
| Applications: | ELISA,ChIP,Dot,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Lysine-specific demethylase 1 regulates differentiation onset and migration of trophoblast stem cells.Zhu D, Holz S, Metzger E, Pavlovic M, Jandausch A, Jilg C, Galgoczy P, Herz C, Moser M, Metzger D, Gunther T, Arnold SJ, Schule R. Nat Commun.2014;5:3174. |