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Data sheet of Histone H3 (K4me1) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Xenopus,Zebra fish |
| Host species | Rabbit |
| Applications | ELISA,ChIP,Dot,WB-Ce |
More info about Histone H3 (K4me1) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB31261 |
| Product name: | Histone H3 (K4me1) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K4me1). |
| Gene id: | 8350 |
| Gene name: | HIST1H3A |
| Gene alias: | H3/A|H3FA |
| Gene description: | histone cluster 1, H3a |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to Histone H3, monomethylated at lysine 4. |
| Protein accession: | P68431 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:100) Western Blot (1:1000) ChIP (1 ug/CHIP) Dot Blot (1:50000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.05% sodium azide). |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Tag: | KLH |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human,Xenopus,Zebra fish |
| Application image: |  |
| Application image note: | ChIP assays were performed using human osteosarcoma (U2OS) cells. A titration of the antibody consisting of 2, 5, 10 or 15 ul per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for the promoter of the ALDOA gene and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). |
| Applications: | ELISA,ChIP,Dot,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | The developmental epigenomics toolbox: ChIP-seq and MethylCap-seq profiling of early zebrafish embryos.Bogdanovi? O, Fernandez-Minan A, Tena JJ, de la Calle-Mustienes E, Gomez-Skarmeta JL. Methods.2013 Aug 15;62(3):207-15. |