Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Human |
Host species | Rabbit |
Applications | ELISA,ChIP,Dot |
Brand: | Abnova |
Reference: | PAB31259 |
Product name: | Histone H2B pan polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Histone H2B pan. |
Gene id: | 8347 |
Gene name: | HIST1H2BC |
Gene alias: | H2B.1|H2B/l|H2BFL|MGC104246|dJ221C16.3 |
Gene description: | histone cluster 1, H2bc |
Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to unmodified sequence at C-terminus of human histone H2B pan. |
Protein accession: | P62807 |
Form: | Liquid |
Recommend dilutions: | ELISA (1:500) ChIP (2 ug/CHIP) Dot Blot (1:20000) The optimal working dilution should be determined by the end user. |
Storage buffer: | In PBS (0.05% sodium azide, 0.05% proclin 300). |
Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Tag: | KLH |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: | |
Application image note: | ChIP assays were performed using human HeLa cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for the inactive MYOD gene. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). |
Applications: | ELISA,ChIP,Dot |
Shipping condition: | Dry Ice |