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Golden Taq Polymerase kit 

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Data sheet of Golden Taq Polymerase kit 

BrandProteoGenix
Product typeProteins
Origin speciesThermus aquaticus
ApplicationsFor routine PCR and RT-PCR: YES For DNA sequencing : NO

More info about Golden Taq Polymerase kit 

Brand: ProteoGenix
Product type: Proteins
Reference: PI-MB001-3000-k
Size: 3000 U
Product name: Golden Taq Polymerase kit 
Product description: The Golden Taq Polymerase, from the hemophilic bacteria Thermus aquaticus is a highly thermostable DNA polymerase used for standard PCR applications in order to amplify DNA sequences. The recombinant form of this enzyme expressed in bacterial expression system (E.coli) has a molecular weight of ~95 kDa. Reaction buffer and an MgSO4 solution are included for possible optimizations
Contains: 3 x 200 µl Taq DNA Polymerase 3 x 1000 µl Reaction Buffer (concentrated 10X) 3 x 800 µl MgSO4 100mM 3 x 100µl dNTPs 10Mm
Origin species: Thermus aquaticus
Our strenghts : Good amplification with minimal optimisation High efficiency of PCR products High sensitivity Good reproductibility
Applications: For routine PCR and RT-PCR: YES For DNA sequencing : NO
Uniprot id: P19821
Expression system: E.coli
Publication: 1: Li Y, Mitaxov V, Waksman G. Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation. Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9491-6. PubMed PMID: 10449720; PubMed Central PMCID: PMC22236. 2: Li Y, Korolev S, Waksman G. Crystal structures of open and closed forms of binary and ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I: structural basis for nucleotide incorporation. EMBO J. 1998 Dec 15;17(24):7514-25. PubMed PMID: 9857206; PubMed Central PMCID: PMC1171095. 3: Li Y, Kong Y, Korolev S, Waksman G. Crystal structures of the Klenow fragment of Thermus aquaticus DNA polymerase I complexed with deoxyribonucleoside triphosphates. Protein Sci. 1998 May;7(5):1116-23. PubMed PMID: 9605316; PubMed Central PMCID: PMC2144016. 4: Eom SH, Wang J, Steitz TA. Structure of Taq polymerase with DNA at the polymerase active site. Nature. 1996 Jul 18;382(6588):278-81. PubMed PMID: 8717047. 5: Ishino Y, Ueno T, Miyagi M, Uemori T, Imamura M, Tsunasawa S, Kato I. Overproduction of Thermus aquaticus DNA polymerase and its structural analysis by ion-spray mass spectrometry. J Biochem. 1994 Nov;116(5):1019-24. PubMed PMID: 7896728. 6: Kim Y, Eom SH, Wang J, Lee DS, Suh SW, Steitz TA. Crystal structure of Thermus aquaticus DNA polymerase. Nature. 1995 Aug 17;376(6541):612-6. PubMed PMID: 7637814. 7: Korolev S, Nayal M, Barnes WM, Di Cera E, Waksman G. Crystal structure of the large fragment of Thermus aquaticus DNA polymerase I at 2.5-A resolution: structural basis for thermostability. Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9264-8. PubMed PMID: 7568114; PubMed Central PMCID: PMC40965. 8: Lawyer FC, Stoffel S, Saiki RK, Myambo K, Drummond R, Gelfand DH. Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus. J Biol Chem. 1989 Apr 15;264(11):6427-37. PubMed PMID: 2649500.
Synonyms: DNA taq polymerase, taq polymerase, taq, DNA polymerase I, thermostable, polA, pol1, Taq polymerase 11
Storage instruction: Store at 4°C for one week. Stable one year at -20°C
Delivery condition: Blue Ice
Delivery lead time in business days: 2-3
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