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Data sheet of Golden Taq Polymerase kit
| Brand | ProteoGenix |
| Product type | Proteins |
| Origin species | Thermus aquaticus |
| Applications | For routine PCR and RT-PCR: YES For DNA sequencing : NO |
More info about Golden Taq Polymerase kit
| Brand: | ProteoGenix |
| Product type: | Proteins |
| Reference: | PI-MB001-250-k |
| Size: | 250 U |
| Product name: | Golden Taq Polymerase kit |
| Product description: | The Golden Taq Polymerase, from the hemophilic bacteria Thermus aquaticus is a highly thermostable DNA polymerase used for standard PCR applications in order to amplify DNA sequences. The recombinant form of this enzyme expressed in bacterial expression system (E.coli) has a molecular weight of ~95 kDa. Reaction buffer and an MgSO4 solution are included for possible optimizations |
| Contains: | 1 x 50 µl Taq DNA Polymerase 1 x 250 µl Reaction buffer (concentrated 10X) 1 x 200 µl MgSO4 100mM 1 x 25µl dNTPs 10Mm |
| Origin species: | Thermus aquaticus |
| Our strenghts : | Good amplification with minimal optimisation High efficiency of PCR products High sensitivity Good reproductibility |
| Applications: | For routine PCR and RT-PCR: YES For DNA sequencing : NO |
| Uniprot id: | P19821 |
| Expression system: | E.coli |
| Publication: | 1: Li Y, Mitaxov V, Waksman G. Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation. Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9491-6. PubMed PMID: 10449720; PubMed Central PMCID: PMC22236. 2: Li Y, Korolev S, Waksman G. Crystal structures of open and closed forms of binary and ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I: structural basis for nucleotide incorporation. EMBO J. 1998 Dec 15;17(24):7514-25. PubMed PMID: 9857206; PubMed Central PMCID: PMC1171095. 3: Li Y, Kong Y, Korolev S, Waksman G. Crystal structures of the Klenow fragment of Thermus aquaticus DNA polymerase I complexed with deoxyribonucleoside triphosphates. Protein Sci. 1998 May;7(5):1116-23. PubMed PMID: 9605316; PubMed Central PMCID: PMC2144016. 4: Eom SH, Wang J, Steitz TA. Structure of Taq polymerase with DNA at the polymerase active site. Nature. 1996 Jul 18;382(6588):278-81. PubMed PMID: 8717047. 5: Ishino Y, Ueno T, Miyagi M, Uemori T, Imamura M, Tsunasawa S, Kato I. Overproduction of Thermus aquaticus DNA polymerase and its structural analysis by ion-spray mass spectrometry. J Biochem. 1994 Nov;116(5):1019-24. PubMed PMID: 7896728. 6: Kim Y, Eom SH, Wang J, Lee DS, Suh SW, Steitz TA. Crystal structure of Thermus aquaticus DNA polymerase. Nature. 1995 Aug 17;376(6541):612-6. PubMed PMID: 7637814. 7: Korolev S, Nayal M, Barnes WM, Di Cera E, Waksman G. Crystal structure of the large fragment of Thermus aquaticus DNA polymerase I at 2.5-A resolution: structural basis for thermostability. Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9264-8. PubMed PMID: 7568114; PubMed Central PMCID: PMC40965. 8: Lawyer FC, Stoffel S, Saiki RK, Myambo K, Drummond R, Gelfand DH. Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus. J Biol Chem. 1989 Apr 15;264(11):6427-37. PubMed PMID: 2649500. |
| Synonyms: | DNA taq polymerase, taq polymerase, taq, DNA polymerase I, thermostable, polA, pol1, Taq polymerase 7 |
| Storage instruction: | Store at 4°C for one week. Stable one year at -20°C |
| Delivery condition: | Blue Ice |
| Delivery lead time in business days: | 2-3 |
| Related products: | - Golden Taq Polymerase kit - Golden Taq Polymerase kit - Golden Taq Polymerase kit |