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Data sheet of Tobacco TEV Protease Recombinant Enzyme
| Brand | ProteoGenix |
| Product type | Proteins |
| Origin species | Tobacco |
| Host species | Escherichia coli (E. coli) |
More info about Tobacco TEV Protease Recombinant Enzyme
| Brand: | ProteoGenix |
| Proteogenix reference: | PX-P1108-100U |
| Protein delivered with tag?: | Yes |
| Size: | 100U |
| Product name: | Tobacco TEV Protease Recombinant Enzyme |
| Fragment type: | Property sequence |
| Origin species: | Tobacco |
| Expression system: | Prokaryotic expression |
| Host species: | Escherichia coli (E. coli) |
| Molecular weight with tag if any: | 54,23 kDa |
| Protein accession: | NP_734212.1 |
| Ncbi reference: | NP_734212.1 |
| Aliases / synonyms: | GST-TEV, NIa-Pro protein (TEV protease) |
| Form: | Frozen |
| Buffer: | 50 mM，pH8.0, 0.5 mM EDTA,DTT 1mM |
| Storage condition: | 4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection) |
| Delivery condition: | Dry Ice |
| Delivery lead time in business days: | 5-7 |
| Related products: | - Human HA-HSP27 Recombinant Protein - Drosophila Hh Recombinant Protein - Human HIRA(155-254) Recombinant Protein |
| Image: |  |
| Description: | TEV (Tobacco Etch Virus) Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus. Due to its high sequence specificity it is regularly used to cleave affinity tags from fusion proteins. The TEV encodes its whole genome as a single massive polyprotein. This is cleaved into functional units by the three proteases: P1 protease (1 cleavage site), helper-component protease (1 cleavage site) and TEV protease (7 cleavage sites). The greatest recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ/S] and cleavage appear between the Gln and Gly/Ser residues. |
| Publications: | 1: MartÃnez F, Daròs JA. Tobacco etch virus protein P1 traffics to the nucleolus and associates with the host 60S ribosomal subunits during infection. J Virol. 2014 Sep;88(18):10725-37. doi: 10.1128/JVI.00928-14. Epub 2014 Jul 2. PubMed PMID: 24991017; PubMed Central PMCID: PMC4178839. 2: Cui X, Wei T, Chowda-Reddy RV, Sun G, Wang A. The Tobacco etch virus P3 protein forms mobile inclusions via the early secretory pathway and traffics along actin microfilaments. Virology. 2010 Feb 5;397(1):56-63. doi: 10.1016/j.virol.2009.11.015. Epub 2009 Nov 30. PubMed PMID: 19945728. 3: Torres-Barceló C, MartÃn S, Daròs JA, Elena SF. From hypo- to hypersuppression: effect of amino acid substitutions on the RNA-silencing suppressor activity of the Tobacco etch potyvirus HC-Pro. Genetics. 2008 Oct;180(2):1039-49. doi: 10.1534/genetics.108.091363. Epub 2008 Sep 9. PubMed PMID: 18780745; PubMed Central PMCID: PMC2567354. 4: Allison RF, Dougherty WG, Parks TD, Willis L, Johnston RE, Kelly M, Armstrong FB. Biochemical analysis of the capsid protein gene and capsid protein of tobacco etch virus: N-terminal amino acids are located on the virion's surface. Virology. 1985 Dec;147(2):309-16. PubMed PMID: 18640560. |