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Data sheet of Mouse IgG2b Isotype Control
| Brand | Exbio |
| Product type | Primary antibodies |
| Clonality | Monoclonal |
More info about Mouse IgG2b Isotype Control
| Brand: | Exbio |
| Product no.: | 11-692-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse IgG2b Isotype Control |
| Clonality: | Monoclonal |
| Negative species: | Human, Mouse, Rat, Other species Not tested |
| Clone: | MPC-11 |
| Isotype: | IgG2b |
| Immunogen: | KLH-coupled trinitrophenol |
| Format: | purified |
| Specificity: | This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues. |
| Categories: | Immunoglobulin Isotype Controls |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished. |
| Usage: | The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *And other., *Liang XS, Zhou Y, Li CZ, Wan MB: Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type-1 of CD8-positive T cells. World J Gastroenterol. 2010 Feb 7;16(5):618-24., *Dewals BG, Vanderplasschen A: Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues. Vet Res. 2011 Aug 22;42(1):95., *Gupta D, Wang Q, Vinson C, Dziarski R: Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1. J Biol Chem. 1999 May 14;274(20):14012-20., *Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK: IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008 May 15;111(10):5037-46. , *Im JS, Tapinos N, Chae GT, Illarionov PA, Besra GS, DeVries GH, Modlin RL, Sieling PA, Rambukkana A, Porcelli SA: Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells. J Immunol. 2006 Oct 15;177(8):5226-35. |
| Related products: | - Mouse Monoclonal to 5-bromodeoxyuridine (BrdU) - Mouse IgG3 Isotype Control - Mouse IgM Isotype Control |
| Shipping condition: | Room temperature |