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Data sheet of Mouse IgG1 Isotype Control
| Brand | Exbio |
| Product type | Primary antibodies |
| Clonality | Monoclonal |
More info about Mouse IgG1 Isotype Control
| Brand: | Exbio |
| Product no.: | 11-457-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse IgG1 Isotype Control |
| Clonality: | Monoclonal |
| Negative species: | Human, Other species Not tested (expected negative), Porcine, Mouse, Rat |
| Clone: | PPV-06 |
| Isotype: | IgG1 |
| Format: | purified |
| Specificity: | This mouse IgG1 monoclonal antibody (clone PPV-06) reacts with undefined epitope on a plant pathogen._x000D_ |
| Categories: | Immunoglobulin Isotype Controls |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-06, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished. |
| Usage: | The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova-Repic A, Stockinger H: Folate Receptor β Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. J Immunol. 2016 Sep 15;197(6):2229-38. |
| Related products: | - Mouse IgG3 Isotype Control - Mouse IgG2a Isotype Control - Mouse IgG2b Isotype Control |
| Shipping condition: | Room temperature |