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Data sheet of Mouse Monoclonal to Syk
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Mouse, Human, Rat, Other not tested |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to Syk
| Brand: | Exbio |
| Product no.: | 11-376-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to Syk |
| Antigen: | Syk |
| Clonality: | Monoclonal |
| Clone: | SYK-01 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant fragment (aa 5-360) of human Syk. |
| Format: | purified |
| Specificity: | The antibody SYK-01 reacts with protein tyrosine kinase p72Syk (Syk; Syk family tyrosine-specific phospho-transferase), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI). |
| Categories: | Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Rodent), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. Elife. 2015 Dec 9;4:e10766., *Heneberg P, Lebduska P, Draberova L, Korb J, Draber P: Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol. 2006 Oct;36(10):2795-806. _x000D_ , *Arudchandran R, Brown MJ, Peirce MJ, Song JS, Zhang J, Siraganian RP, Blank U, Rivera J: The Src homology 2 domain of Vav is required for its compartmentation to the plasma membrane and activation of c-Jun NH(2)-terminal kinase 1. J Exp Med. 2000 Jan 3;191(1):47-60. _x000D_ , *Halova I, Draberova L, Draber P: A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fc epsilon receptor. Int Immunol. 2002 Feb;14(2):213-23. _x000D_ |
| General references: | *Parravicini V, Gadina M, Kovarova M, Odom S, Gonzalez-Espinosa C, Furumoto Y, Saitoh S, Samelson LE, O'Shea JJ, Rivera J: Fyn kinase initiates complementary signals required for IgE-dependent mast cell degranulation. Nat Immunol. 2002 Aug;3(8):741-8. Epub 2002 Jul 1. _x000D_ , *Manetz TS, Gonzalez-Espinosa C, Arudchandran R, Xirasagar S, Tybulewicz V, Rivera J: Vav1 regulates phospholipase cgamma activation and calcium responses in mast cells. Mol Cell Biol. 2001 Jun;21(11):3763-74. _x000D_ , *Kovarova M, Tolar P, Arudchandran R, Draberova L, Rivera J, Draber P: Structure-function analysis of Lyn kinase association with lipid rafts and initiation of early signaling events after Fcepsilon receptor I aggregation. Mol Cell Biol. 2001 Dec;21(24):8318-28. _x000D_ , *Swann PG, Odom S, Zhou YJ, Szallasi Z, Blumberg PM, Draber P, Rivera J: Requirement for a negative charge at threonine 60 of the FcRgamma for complete activation of Syk. J Biol Chem. 1999 Aug 13;274(33):23068-77. _x000D_ , *Ma H, Yankee TM, Hu J, Asai DJ, Harrison ML, Geahlen RL: Visualization of Syk-antigen receptor interactions using green fluorescent protein: differential roles for Syk and Lyn in the regulation of receptor capping and internalization. J Immunol. 2001 Feb 1;166(3):1507-16. , *Amoui M, Draberova L, Tolar P, Draber P: Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors. Eur J Immunol. 1997 Jan;27(1):321-8. _x000D_ , *Tolar P, Draberova L, Draber P: Protein tyrosine kinase Syk is involved in Thy-1 signaling in rat basophilic leukemia cells._x000D_ Eur J Immunol. 1997 Dec;27(12):3389-97. _x000D_ |
| Related products: | - Mouse Monoclonal to TCR beta (Vbeta5.3-related) - Mouse Monoclonal to TCR alpha/beta - Mouse Monoclonal to TCR alpha/beta (rat) |
| Shipping condition: | Room temperature |