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Data sheet of Mouse Monoclonal to Perforin
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Human, Bovine |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to Perforin
| Brand: | Exbio |
| Product no.: | 11-765-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to Perforin |
| Antigen: | Perforin |
| Clonality: | Monoclonal |
| Negative species: | Mouse |
| Clone: | dG9 |
| Isotype: | IgG2b |
| Immunogen: | purified granules from human YT lymphoma cell line |
| Format: | purified |
| Specificity: | The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells. |
| Categories: | Apoptosis (Veterinary), Apoptosis (Human) |
| Concentration: | 1 mg/ml |
| Storage buffer: | Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *Hameed A, Olsen KJ, Cheng L, Fox WM, Hruban RH, Podack ER: Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am J Pathol. 1992 May;140(5):1025-30., *Hersperger AR, Makedonas G, Betts MR: Flow cytometric detection of perforin upregulation in human CD8 T cells. Cytometry A. 2008 Nov;73(11):1050-7., *Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW: Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions. PLoS Pathog. 2012;8(11):e1002984., *Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. J Mol Cell Biol. 2010 Jun;2(3):139-51., *Vargas-Inchaustegui DA, Demberg T, Robert-Guroff M: A CD8α(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities. Immunology. 2011 Nov;134(3):326-40. |
| Related products: | - Mouse Monoclonal to Placental alkaline phosphatase - Mouse Monoclonal to Phosphotyrosine - Mouse Monoclonal to PKAc |
| Shipping condition: | Room temperature |