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Data sheet of Mouse Monoclonal to PAG / Cbp
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Mouse, Human, Rat, Bovine, Other not tested |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to PAG / Cbp
| Brand: | Exbio |
| Product no.: | 11-346-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to PAG / Cbp |
| Antigen: | PAG / Cbp |
| Clonality: | Monoclonal |
| Clone: | PAG-C1 |
| Isotype: | IgG2b |
| Immunogen: | C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH. |
| Format: | purified |
| Specificity: | The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. |
| Categories: | Adaptor Proteins (Veterinary), Adaptor Proteins (Rodent), Adaptor Proteins (Human), Adaptor Proteins |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *Vang T, Abrahamsen H, Myklebust S, Horejsi V, Tasken K: Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling. J Biol Chem. 2003 May 16;278(20):17597-600. , *Davidson D, Bakinowski M, Thomas ML, Horejsi V, Veillette A: Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor._x000D_ Mol Cell Biol. 2003 Mar;23(6):2017-28. _x000D_ , *Baumgartner M, Angelisova P, Setterblad N, Mooney N, Werling D, Horejsi V, Langsley G: Constitutive exclusion of Csk from Hck-positive membrane microdomains permits_x000D_ Src kinase-dependent proliferation of Theileria-transformed B lymphocytes. Blood. 2003 Mar 1;101(5):1874-81. _x000D_ |
| General references: | *Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17(1):35-49., *Horejsà V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16. |
| Related products: | - Mouse Monoclonal to PDE8a - Mouse Monoclonal to PCLO - Mouse Monoclonal to PCNA |
| Shipping condition: | Room temperature |