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Data sheet of Mouse Monoclonal to NHERF1 / EBP50
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Other not tested, Human |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to NHERF1 / EBP50
| Brand: | Exbio |
| Product no.: | 11-348-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to NHERF1 / EBP50 |
| Antigen: | NHERF1 / EBP50 |
| Clonality: | Monoclonal |
| Clone: | EBP-10 |
| Isotype: | IgG2b |
| Immunogen: | Bacterially produced recombinant full-length human NHERF1. |
| Format: | purified |
| Specificity: | The antibody EBP-10 reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein. |
| Categories: | Cytoskeleton: Actin System, Cytoskeleton: Actin System (Human), Adaptor Proteins (Human), Adaptor Proteins |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| General references: | *Lederer ED, Khundmiri SJ, Weinman E.J.: Role of NHERF-1 in regulation of the activity of Na-K ATPase and sodium-phosphate co-transport in epithelial cells. J Am Soc Nephrol. 2003 Jul;14(7):1711-9._x000D_ _x000D_ _x000D_ , *Weinman EJ, Cunningham R, Wade JB, Shenolikar S.: The role of NHERF-1 in the regulation of renal proximal tubule sodium-hydrogen exchanger 3 and sodium-dependent phosphate cotransporter 2a. J Physiol. 2005 Aug 15;567(Pt 1):27-32._x000D_ _x000D_ , *Wheeler D, Sneddon WB, Wang B, Friedman PA, Romero G.: NHERF-1 and the cytoskeleton regulate the traffic and membrane dynamics of G protein-coupled receptors. J Biol Chem. 2007 Aug 24;282(34):25076-87._x000D_ _x000D_ , *Morales FC, Takahashi Y, Momin S, Adams H, Chen X, Georgescu M.M.: NHERF1/EBP50 head-to-tail intramolecular interaction masks association with PDZ_x000D_ domain ligands. Mol Cell Biol. 2007 Apr;27(7):2527-37._x000D_ _x000D_ , *Kreimann EL, Morales FC, de Orbeta-Cruz J, Takahashi Y, Adams H, Liu TJ, McCrea PD, Georgescu M.M.: Cortical stabilization of beta-catenin contributes to HERF1/EBP50 tumor suppressor function. Oncogene. 2007 Aug 9;26(36):5290-9._x000D_ _x000D_ , *Brdickova N, Brdicka T, Andera L, Spicka J, Angelisova P, Milgram SL, Horejsi V.: Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton. FEBS Lett. 2001 Oct 26;507(2):133-6. _x000D_ |
| Related products: | - Mouse Monoclonal to Notch 1 - Mouse Monoclonal to Nitrotyrosine - Mouse Monoclonal to NK1.1 / CD161bc (mouse) |
| Shipping condition: | Room temperature |