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Data sheet of Mouse Monoclonal to Neurofilament heavy protein
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Human, Porcine, Rat |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to Neurofilament heavy protein
| Brand: | Exbio |
| Product no.: | 11-440-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to Neurofilament heavy protein |
| Antigen: | Neurofilament heavy protein |
| Clonality: | Monoclonal |
| Negative species: | Mouse |
| Clone: | NF-05 |
| Isotype: | IgG1 |
| Immunogen: | Pig brain neurofilament protein-enriched fraction after depolymerization of microtubules |
| Format: | purified |
| Specificity: | The antibody NF-05 recognizes a nonphosphorylated epitope of neurofilament heavy protein (NF-H), a 210 kDa intracellular structural protein of Intermediate Filament Proteins family. NF-H is mainly expressed in the central and peripheral nervous system and reproductive system and is biochemically very stable. _x000D_ |
| Categories: | Cytoskeleton: Intermediate Filaments (Veterinary), Cytoskeleton: Intermediate Filaments (Rodent), Neurobiology (Veterinary), Neurobiology (Rodent), Neurobiology (Human), Cytoskeleton: Intermediate Filaments (Human), Cytoskeleton: Intermediate Filaments |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | Neurofilaments (NFs) are a type of intermediate filaments (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights – neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by precipitation and chromatography |
| Product specific references: | *Porchet R, Probst A, Draberova E, Draber P, Riederer IM, Riederer BM: Differential subcellular localization of phosphorylated neurofilament and tau proteins in degenerating neurons of the human entorhinal cortex._x000D_ Neuroreport. 2003 May 23;14(7):929-33. _x000D_ |
| General references: | *Cote F, Collard JF, Julien JP: Progressive neuronopathy in transgenic mice expressing the human neurofilament_x000D_ heavy gene: a mouse model of amyotrophic lateral sclerosis. Cell. 1993 Apr 9;73(1):35-46. _x000D_ , *Ohara O, Gahara Y, Miyake T, Teraoka H, Kitamura T:_x000D_ Neurofilament deficiency in quail caused by nonsense mutation in neurofilament-L gene. J Cell Biol. 1993 Apr;121(2):387-95. _x000D_ , *Brown RH Jr.: Amyotrophic lateral sclerosis: recent insights from genetics and transgenic mice. Cell. 1995 Mar 10;80(5):687-92., *Petzold A, Keir G, Warren J, Fox N, Rossor MN: A systematic review and meta-analysis of CSF neurofilament protein levels as biomarkers in dementia. Neurodegener Dis. 2007;4(2-3):185-94._x000D_ _x000D_ _x000D_ , *Miyazawa I, Nakashima I, Petzold A, Fujihara K, Sato S, Itoyama Y: High CSF neurofilament heavy chain levels in neuromyelitis optica. Neurology. 2007 Mar 13;68(11):865-7._x000D_ _x000D_ _x000D_ , *Wild EJ, Petzold A, Keir G, Tabrizi SJ: Plasma neurofilament heavy chain levels in Huntington's disease._x000D_ Neurosci Lett. 2007 May 7;417(3):231-3._x000D_ |
| Related products: | - Mouse Monoclonal to NG2 / Chondroitin sulfate proteoglycan 4 - Mouse Monoclonal to Neurofilament heavy protein - Mouse Monoclonal to Neurofilament medium protein |
| Shipping condition: | Room temperature |