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Data sheet of Rabbit Polyclonal to MRCK alpha
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Human |
| Clonality | Polyclonal |
More info about Rabbit Polyclonal to MRCK alpha
| Brand: | Exbio |
| Product no.: | 11-636-C100 |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Product name: | Rabbit Polyclonal to MRCK alpha |
| Antigen: | MRCK alpha |
| Clonality: | Polyclonal |
| Clone: | Polyclonal |
| Immunogen: | Peptide corresponding to the amino acids 1671-1685 of human MRCK alpha |
| Format: | purified |
| Specificity: | The polyclonal antibody anti-MRCK alpha recognizes myotonic dystrophy kinase-related Cdc42-binding kinase alpha, a 180 kDa Ser/Thr kinase, which is responsible for reorganization of actin cytoskeleton. |
| Categories: | Cytoskeleton: Actin System (Human), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human) |
| Concentration: | 0.25 mg/ml |
| Storage buffer: | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide. |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | MRCK alpha (myotonic dystrophy kinase-related Cdc42-binding kinase alpha) is a member of the dystrophia myotonica protein kinase (DMPK) family that functions downstream of Cdc42 in actin cytoskeletal reorganization. It is a serine/threonine kinase with multiple functional domains, including phorbol ester-responsive C1 domain. Three independent coiled-coil domains and the N-terminal region preceding the kinase domain are responsible for intermolecular interactions leading to MRCK alpha multimerization. The transautophosphorylation process is critical for regulation of MRCK alpha catalytic activities. Binding of phorbol esters to MRCK releases its autoinhibition, allowing N-terminal dimerization and subsequent kinase activation. |
| Purity: | > 95% (by SDS-PAGE) before adding BSA |
| Purification: | Purified from rabbit serum by affinity chromatography |
| Product specific references: | *Cmejla R, Ptackova P, Petrak J, Savvulidi F, Cerny J, Sebesta O, Vyoral D: Human MRCKalpha is regulated by cellular iron levels and interferes with transferrin iron uptake. Biochem Biophys Res Commun. 2010 Apr 30;395(2):163-7. |
| General references: | *Tan I, Seow KT, Lim L, Leung T: Intermolecular and intramolecular interactions regulate catalytic activity of_x000D_ myotonic dystrophy kinase-related Cdc42-binding kinase alpha. Mol Cell Biol. 2001 Apr;21(8):2767-78., *Tan I, Ng CH, Lim L, Leung T: Phosphorylation of a novel myosin binding subunit of protein phosphatase 1 reveals a conserved mechanism in the regulation of actin cytoskeleton. J Biol Chem. 2001 Jun 15;276(24):21209-16., *Choi SH, Czifra G, Kedei N, Lewin NE, Lazar J, Pu Y, Marquez VE, Blumberg PM: Characterization of the interaction of phorbol esters with the C1 domain of MRCK (myotonic dystrophy kinase-related Cdc42 binding kinase) alpha/beta. J Biol Chem. 2008 Apr 18;283(16):10543-9. |
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| Shipping condition: | Room temperature |