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Data sheet of Rabbit Polyclonal to MEK 1/2 / MAPKK 1/2
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Human |
| Clonality | Polyclonal |
More info about Rabbit Polyclonal to MEK 1/2 / MAPKK 1/2
| Brand: | Exbio |
| Product no.: | 11-508-C050 |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Product name: | Rabbit Polyclonal to MEK 1/2 / MAPKK 1/2 |
| Antigen: | MEK 1/2 |
| Clonality: | Polyclonal |
| Clone: | Polyclonal |
| Immunogen: | Synthetic peptide (coupled with KLH) derived from aminoacid sequence 33-53 of human MAPK Kinase 1. The immunogen sequence is highly conserved within species (human, mouse, rat, hamster, bovine etc.). |
| Format: | purified |
| Specificity: | The polyclonal antibody reacts with MEK 1/2. MEK 1 and MEK 2 are integral components of the MAP kinase cascade, an important pathway for cell growth and differentiation._x000D_ _x000D_ _x000D_ |
| Categories: | Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) |
| Concentration: | 1 mg/ml |
| Storage buffer: | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide. |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | MEK (MAPKK, kinase of mitogen-activated protein kinase) is the medium one of three components of MAP kinase cascade – an important signaling pathway that regulates cell growth and differentiation. Raf (MAPKKK) activates MEK 1 and 2 via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK (MAPK, mitogen-activated protein kinase). This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade. Phosphorylated ERK is capable of translocating to the nucleus, where it regulates gene expression by activating transcription factors._x000D_ _x000D_ |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from rabbit serum by affinity chromatography |
| Product specific references: | *Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15(11):909-912. |
| General references: | *Park ER, Eblen ST, Catling AD: MEK1 activation by PAK: a novel mechanism. Cell Signal. 2007 Jul;19(7):1488-96. _x000D_ _x000D_ , *Scholl FA, Dumesic PA, Barragan DI, Harada K, Bissonauth V, Charron J, Khavari PA. Mek1/2 MAPK kinases are essential for Mammalian development, homeostasis, and Raf-induced hyperplasia. Dev Cell. 2007 Apr;12(4):615-29._x000D_ _x000D_ _x000D_ , *McCubrey JA, Steelman LS, Chappell WH, Abrams SL, Wong EW, Chang F, Lehmann B, Terrian DM, Milella M, Tafuri A, Stivala F, Libra M, Basecke J, Evangelisti C,_x000D_ Martelli AM, Franklin RA. Roles of the Raf/MEK/ERK pathway in cell growth, malignant transformation and_x000D_ drug resistance. Biochim Biophys Acta. 2007 Aug;1773(8):1263-84._x000D_ _x000D_ , *Sundaram MV: RTK/Ras/MAPK signaling. WormBook. 2006 Feb 11;:1-19._x000D_ _x000D_ _x000D_ , *Park JI, Strock CJ, Ball DW, Nelkin BD: The Ras/Raf/MEK/extracellular signal-regulated kinase pathway induces autocrine-paracrine growth inhibition via the leukemia inhibitory factor/JAK/STAT pathway. Mol Cell Biol. 2003 Jan;23(2):543-54._x000D_ _x000D_ _x000D_ , *Chang F, Steelman LS, Lee JT, Shelton JG, Navolanic PM, Blalock WL, Franklin RA, McCubrey JA: Signal transduction mediated by the Ras/Raf/MEK/ERK pathway from cytokine receptors to transcription factors: potential targeting for therapeutic intervention. Leukemia. 2003 Jul;17(7):1263-93._x000D_ _x000D_ _x000D_ |
| Related products: | - Rabbit Polyclonal to Mycobacterium tuberculosis antigen Acr2 - Rabbit Polyclonal to MRCK alpha - Rabbit Polyclonal to Mycobacterium tuberculosis antigen Acr1 |
| Shipping condition: | Room temperature |