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Data sheet of Mouse Monoclonal to LIME
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Human |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to LIME
| Brand: | Exbio |
| Product no.: | 11-401-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to LIME |
| Antigen: | LIME |
| Clonality: | Monoclonal |
| Clone: | LIME-10 |
| Isotype: | IgG2a |
| Immunogen: | COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin. |
| Format: | purified |
| Specificity: | The antibody LIME-10 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells. |
| Categories: | Adaptor Proteins (Human), Adaptor Proteins |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rudiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsi V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers._x000D_ Blood. 2006 Jan 1;107(1):213-21. _x000D_ , *Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsà V. LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8_x000D_ coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62._x000D_ |
| General references: | *Horejsà V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16., *Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17(1):35-49. |
| Related products: | - Mouse Monoclonal to Lyn - Mouse Monoclonal to LIME (mouse) - Mouse Monoclonal to LST1 |
| Shipping condition: | Room temperature |