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Data sheet of Mouse Monoclonal to GRAP2/GADS
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Human |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to GRAP2/GADS
| Brand: | Exbio |
| Product no.: | 11-639-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to GRAP2/GADS |
| Antigen: | GRAP2/GADS |
| Clonality: | Monoclonal |
| Clone: | UW40 |
| Isotype: | IgG2a |
| Immunogen: | GST-fusion human GRAP2/GADS protein |
| Format: | purified |
| Specificity: | The mouse monoclonal antibody UW40 recognizes GRAP2/GADS, a 41 kDa cytoplasmic adaptor protein that plays a pivotal role during the early events of signal transduction in T cells. |
| Categories: | Adaptor Proteins (Human) |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | GRAP2/GADS (Grb2-related adaptor protein 2 / Grb2-related adaptor downstream of Shc) is a cytoplasmic adaptor protein containing N- and C-terminal SH3 domains flanking a central SH2 domain and a proline/glutamine-rich region. It is expressed predominantly in lymphoid tissue and hematopoietic cells, particularly in T cells. GRAP2/GADS plays a pivotal role during the early events of T cell signal transduction by recruiting the adaptor protein SLP-76 and its associated molecules, such as Vav, Nck, Itk, and ADAP, to the transmembrane adaptor protein LAT. GRAP2/GADS also binds several other signaling proteins, namely Gab2, HPK1 (hematopoietic progenitor kinase 1), and Cbl. Unlike similar adaptor protein Grb2, GRAP2/GADS shows higher selectivity when binding to the particular phosphorylated tyrosines of LAT adaptor. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15(11):909-912., *Yankee TM, Yun TJ, Draves KE, Ganesh K, Bevan MJ, Murali-Krishna K, Clark EA: The Gads (GrpL) adaptor protein regulates T cell homeostasis. J Immunol. 2004 Aug 1;173(3):1711-20., *Yankee TM, Draves KE, Ewings MK, Clark EA, Graves JD: CD95/Fas induces cleavage of the GrpL/Gads adaptor and desensitization of antigen receptor signaling. Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6789-93. |
| General references: | *Dalheimer SL, Zeng L, Draves KE, Hassaballa A, Jiwa NN, Parrish TD, Clark EA, Yankee TM: Gads-deficient thymocytes are blocked at the transitional single positive CD4+_x000D_ stage. Eur J Immunol. 2009 May;39(5):1395-404., *Cho S, Velikovsky CA, Swaminathan CP, Houtman JC, Samelson LE, Mariuzza RA: Structural basis for differential recognition of tyrosine-phosphorylated sites in_x000D_ the linker for activation of T cells (LAT) by the adaptor Gads. EMBO J. 2004 Apr 7;23(7):1441-51, *Watanabe R, Harada Y, Takeda K, Takahashi J, Ohnuki K, Ogawa S, Ohgai D, Kaibara N, Koiwai O, Tanabe K, Toma H, Sugamura K, Abe R: Grb2 and Gads exhibit different interactions with CD28 and play distinct roles in_x000D_ CD28-mediated costimulation. J Immunol. 2006 Jul 15;177(2):1085-91., *Moran O, Roessle MW, Mariuzza RA, Dimasi N: Structural features of the full-length adaptor protein GADS in solution_x000D_ determined using small-angle X-ray scattering. Biophys J. 2008 Mar 1;94(5):1766-72. |
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| Shipping condition: | Room temperature |