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Data sheet of Rabbit Polyclonal to GFP / Green Fluorescent Protein
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Recognizes fusion proteins in all species |
| Clonality | Polyclonal |
More info about Rabbit Polyclonal to GFP / Green Fluorescent Protein
| Brand: | Exbio |
| Product no.: | 11-476-C025 |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Product name: | Rabbit Polyclonal to GFP / Green Fluorescent Protein |
| Antigen: | GFP |
| Clonality: | Polyclonal |
| Clone: | Polyclonal |
| Immunogen: | EGFP, a native full-length protein_x000D_ |
| Format: | purified |
| Specificity: | The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species._x000D_ |
| Categories: | Epitope Tags |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (λ=509 nm) when excited (excitation by Blue or UV light, absorption peak λ=395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chomophore mutations have been created. The EnhancedGFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. _x000D_ Antibodies raised against full-length GFP variants should also detect other variants of the protein. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from rabbit serum by affinity chromatography |
| Product specific references: | *Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun 7;25(11):2326-37. Epub 2006 May 25. |
| Related products: | - Rabbit Polyclonal to LIME - Rabbit Polyclonal to Grb2 - Rabbit Polyclonal to Kinesin |
| Shipping condition: | Room temperature |