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Data sheet of Mouse Monoclonal to GAPDHS
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Human, Porcine |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to GAPDHS
| Brand: | Exbio |
| Product no.: | 11-301-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to GAPDHS |
| Antigen: | GAPDHS |
| Clonality: | Monoclonal |
| Clone: | Hs-8 |
| Isotype: | IgM |
| Immunogen: | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
| Format: | purified |
| Specificity: | The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, previously identified under the general name "intra-acrosomal protein"._x000D_ |
| Categories: | Fertility & Reproduction (Veterinary), Fertility & Reproduction (Human), Fertility & Reproduction |
| Concentration: | 1 mg/ml |
| Storage buffer: | Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by precipitation and chromatography |
| General references: | *Peknicova J, Kyselova V, Buckiova D, Boubelik M: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May;47(5):311-8. _x000D_ , *Chladek D, Peknicova J, Capkova J, Geussova G, Tepla O, Madar J: Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization. Ceska Gynekol. 2000 Jan;65(1):28-32. Czech. _x000D_ , *Teplá O. et al. In: In vitro Fertilization & Human Reproductive Genetics, ed. Monduzzi, Bologna, 107, (1999). |
| Related products: | - Mouse Monoclonal to GFAP - Mouse Monoclonal to GARP / LRRC32 - Mouse Monoclonal to GCPII / PSMA |
| Shipping condition: | Room temperature |