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Data sheet of Mouse Monoclonal to gamma-tubulin 1
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Human, Porcine, Mouse, Rat, Bovine, Chicken, Protozoa, Plants |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to gamma-tubulin 1
| Brand: | Exbio |
| Product no.: | 11-465-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to gamma-tubulin 1 |
| Antigen: | gamma-tubulin 1 |
| Clonality: | Monoclonal |
| Clone: | TU-30 |
| Isotype: | IgG1 |
| Immunogen: | C-terminal peptide of gamma-tubulin counjugated to KLH. |
| Format: | purified |
| Specificity: | The antibody TU-30 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the amino acid sequence TRPDYI (aa439-444 in human), which is present on human gamma-tubulin 1 but not on human gamma-tubulin 2. |
| Categories: | Cytoskeleton: Tubulin System (Veterinary), Cytoskeleton: Tubulin System (Rodent), Cytoskeleton: Tubulin System (Human), Cytoskeleton: Microtubular System |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface. Humans possess two gamma-tubulin genes. Gamma-tubulin 1 represents a ubiquitous isotype, whereas gamma-tubulin 2 is found predominantly in the brain, where it may be endowed with divergent functions beyond microtubule nucleation._x000D_ _x000D_ |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *Černohorská M, Sulimenko V, Hájková Z, Sulimenko T, Sládková V, Vinopal S, Dráberová E, Dráber P: GIT1/βPIX signaling proteins and PAK1 kinase regulate microtubule nucleation. Biochim Biophys Acta. 2016 Jun;1863(6 Pt A):1282-97., *Katsetos CD, Reddy G, Dráberová E, Smejkalová B, Del Valle L, Ashraf Q, Tadevosyan A, Yelin K, Maraziotis T, Mishra OP, Mörk S, Legido A, Nissanov J, Baas PW, de Chadarévian JP, Dráber P: Altered cellular distribution and subcellular sorting of gamma-tubulin in diffuse astrocytic gliomas and human glioblastoma cell lines. J Neuropathol Exp Neurol. 2006 May;65(5):465-77., *Dráberová E, Sulimenko V, Vinopal S, Sulimenko T, Sládková V, D'Agostino L, Sobol M, Hozák P, Křen L, Katsetos CD, Dráber P: Differential expression of human γ-tubulin isotypes during neuronal development and oxidative stress points to a γ-tubulin-2 prosurvival function. FASEB J. 2017 May;31(5):1828-1846., *Novakova M, Draberova E, Schurmann W, Czihak G, Viklicky V, Draber P: gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies. Cell Motil Cytoskeleton. 1996;33(1):38-51. _x000D_ , *Binarova P, Cenklova V, Hause B, Kubatova E, Lysak M, Dolezel J, Bogre L, Draber P: Nuclear gamma-tubulin during acentriolar plant mitosis. Plant Cell. 2000 Mar;12(3):433-42. _x000D_ , *Libusova L, Sulimenko T, Sulimenko V, Hozak P, Draber P: gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms. Exp Cell Res. 2004 May 1;295(2):375-86. _x000D_ |
| General references: | *Sulimenko V, Dráberová E, Sulimenko T, Macurek L, Richterová V, Dráber P, Dráber P: Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases. J Immunol. 2006 Jun 15;176(12):7243-53., *Wiese C, Zheng Y: Microtubule nucleation: gamma-tubulin and beyond. J Cell Sci. 2006 Oct 15;119(Pt 20):4143-53., *Draberova L, Draberova E, Surviladze Z, Draber P, Draber P: Protein tyrosine kinase p53/p56 (lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells. Int Immunol. 1999 Nov;11(11):1829-39. _x000D_ , *Haren L, Remy MH, Bazin I, Callebaut I, Wright M, Merdes A._x000D_ NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly. J Cell Biol. 2006 Feb 13;172(4):505-15. |
| Related products: | - Mouse Monoclonal to GARP / LRRC32 - Mouse Monoclonal to Gamma-tubulin complex component 2 - Mouse Monoclonal to GAPDHS |
| Shipping condition: | Room temperature |