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Data sheet of Mouse Monoclonal to CD8
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Human |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to CD8
| Brand: | Exbio |
| Product no.: | 11-207-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to CD8 |
| Antigen: | CD8 |
| Clonality: | Monoclonal |
| Clone: | MEM-31 |
| Isotype: | IgG2a |
| Immunogen: | Crude thymus membrane fraction. |
| Format: | purified |
| Specificity: | The antibody MEM-31 recognizes a conformationally-dependent epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa). The antibody does not react with formaldehyde-fixed cells; negative in Western Blotting application. HLDA III; WS Code T 575 |
| Categories: | CD and Related Antigens (Human) |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15(4):1246-61., *Horejsi V et al.: Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen._x000D_ Folia Biol. (Praha) 32, 12 (1986). , *Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by_x000D_ the (-1) frame of U79260(FTO). J Biomed Biotechnol. 2010;2010:841451., *EstefanÃa E, Flores R, Gómez-Lozano N, Aguilar H, López-Botet M, Vilches C: Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets. J Immunol. 2007 Apr 1;178(7):4402-10., *Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84., *Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsà V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62., *Horejsà V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel_x000D_ broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha). 1988;34(1):23-34._x000D_ _x000D_ _x000D_ , *Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987). |
| General references: | *van den Berg HA, Wooldridge L, Laugel B, Sewell AK: Coreceptor CD8-driven modulation of T cell antigen receptor specificity. J Theor Biol. 2007 Nov 21;249(2):395-408._x000D_ _x000D_ , *Pang DJ, Hayday AC, Bijlmakers MJ.: CD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, Not CD8alpha alpha homodimers. J Biol Chem. 2007 May 4;282(18):13884-94._x000D_ _x000D_ , *Devine L, Thakral D, Nag S, Dobbins J, Hodsdon ME, Kavathas PB: Mapping the binding site on CD8 beta for MHC class I reveals mutants with enhanced binding. J Immunol. 2006 Sep 15;177(6):3930-8._x000D_ _x000D_ _x000D_ |
| Related products: | - Mouse Monoclonal to CD82 - Mouse Monoclonal to CD80 / B7-1 - Mouse Monoclonal to CD81 |
| Shipping condition: | Room temperature |