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Data sheet of Mouse Monoclonal to CD47 / Integrin Associated Protein
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Non-Human Primates, Human, Porcine |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to CD47 / Integrin Associated Protein
| Brand: | Exbio |
| Product no.: | 11-225-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to CD47 / Integrin Associated Protein |
| Antigen: | CD47 |
| Clonality: | Monoclonal |
| Clone: | MEM-122 |
| Isotype: | IgM |
| Immunogen: | COS-7 (African green monkey) cells |
| Format: | purified |
| Specificity: | The antibody MEM-122 reacts with CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. _x000D_ HLDA VI; WS Code AS A051 |
| Categories: | CD Antigens & MHC (Veterinary), CD and Related Antigens (Human) |
| Concentration: | 1 mg/ml |
| Storage buffer: | Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by precipitation and chromatography |
| Product specific references: | *Smith RE, Patel V, Seatter SD, Deehan MR, Brown MH, Brooke GP, Goodridge HS, Howard CJ, Rigley KP, Harnett W, Harnett MM: A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes. Blood. 2003 Oct 1;102(7):2532-40., *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). |
| General references: | *Isenberg JS, Pappan LK, Romeo MJ, Abu-Asab M, Tsokos M, Wink DA, Frazier WA, Roberts DD: Blockade of thrombospondin-1-CD47 interactions prevents necrosis of full thickness skin grafts. Ann Surg. 2008 Jan;247(1):180-90._x000D_ _x000D_ _x000D_ , *Orazizadeh M, Lee HS, Groenendijk BC, Millward-Sadler J, Wright MO, Lindberg FP, Salter DM. CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model. Arthritis Res Ther. 2008 Jan 10;10(1):R4 _x000D_ _x000D_ , *Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H: Role for CD47-SIRPalpha signaling in xenograft rejection by macrophages. Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5062-6._x000D_ _x000D_ , *Murata T, Ohnishi H, Okazawa H, Murata Y, Kusakari S, Hayashi Y, Miyashita M, Itoh H, Oldenborg PA, Furuya N, Matozaki T: CD47 promotes neuronal development through Src- and FRG/Vav2-mediated activation _x000D_ of Rac and Cdc42. J Neurosci. 2006 Nov 29;26(48):12397-407._x000D_ _x000D_ _x000D_ , *Barazi HO, Li Z, Cashel JA, Krutzsch HC, Annis DS, Mosher DF, Roberts DD: Regulation of integrin function by CD47 ligands. Differential effects on alpha_x000D_ vbeta 3 and alpha 4beta1 integrin-mediated adhesion. J Biol Chem. 2002 Nov 8;277(45):42859-66._x000D_ _x000D_ |
| Related products: | - Mouse Monoclonal to CD49c / Integrin alpha 3 - Mouse Monoclonal to CD48 / BLAST-1 - Mouse Monoclonal to CD49b / Integrin alpha 2 |
| Shipping condition: | Room temperature |