Cart 0 Product Products (empty)
No products
To be determined Shipping
0,00 € Total
Prices are tax excluded
Product successfully added to your shopping cart
Quantity
Total
There are 0 items in your cart. There is 1 item in your cart.
Total products (tax excl.)
Total shipping (tax excl.) To be determined
Total (tax excl.)
Data sheet of Rat Monoclonal to CD25 (mouse) / IL-2R alpha chain
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Mouse |
| Clonality | Monoclonal |
More info about Rat Monoclonal to CD25 (mouse) / IL-2R alpha chain
| Brand: | Exbio |
| Product no.: | 11-614-C100 |
| Product type: | Primary antibodies |
| Host species: | Rat |
| Product name: | Rat Monoclonal to CD25 (mouse) / IL-2R alpha chain |
| Antigen: | CD25 (mouse) |
| Clonality: | Monoclonal |
| Clone: | PC61.5 |
| Isotype: | IgG1 |
| Immunogen: | B6.1 CTL cell line |
| Format: | purified |
| Specificity: | The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes. |
| Categories: | CD and Related Antigens & MHC (Rodent) |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *Tenorio EP, OlguÃn JE, Fernández J, Vieyra P, Saavedra R: Reduction of Foxp3+ cells by depletion with the PC61 mAb induces mortality in resistant BALB/c mice infected with Toxoplasma gondii. J Biomed Biotechnol. 2010;2010:786078., *Wei HX, Chuang YH, Li B, Wei H, Sun R, Moritoki Y, Gershwin ME, Lian ZX, Tian Z: CD4+ CD25+ Foxp3+ regulatory T cells protect against T cell-mediated fulminant hepatitis in a TGF-beta-dependent manner in mice. J Immunol. 2008 Nov 15;181(10):7221-9., *Yu CT, Feng MH, Shih HM, Lai MZ: Increased p300 expression inhibits glucocorticoid receptor-T-cell receptor_x000D_ antagonism but does not affect thymocyte positive selection. Mol Cell Biol. 2002 Jul;22(13):4556-66., *Aiba Y, Hirayama F, Ogawa M: Clonal proliferation and cytokine requirement of murine progenitors for natural_x000D_ killer cells. Blood. 1997 Jun 1;89(11):4005-12., *Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL-2, which is required for CD(4+)CD25+ T cell regulation of_x000D_ effector CD8+ T cell development for contact hypersensitivity responses. J Leukoc Biol. 2005 Sep;78(3):725-35., *Van VQ, Darwiche J, Raymond M, Lesage S, Bouguermouh S, Rubio M, Sarfati M: Cutting edge: CD47 controls the in vivo proliferation and homeostasis of peripheral CD4+ CD25+ Foxp3+ regulatory T cells that express CD103. J Immunol. 2008 Oct 15;181(8):5204-8., *Kotner J, Tarleton R: Endogenous CD4(+) CD25(+) regulatory T cells have a limited role in the control of Trypanosoma cruzi infection in mice. Infect Immun. 2007 Feb;75(2):861-9., *Jones CM, Cose SC, Coles RM, Winterhalter AC, Brooks AG, Heath WR, Carbone FR: Herpes simplex virus type 1-specific cytotoxic T-lymphocyte arming occurs within lymph nodes draining the site of cutaneous infection. J Virol. 2000 Mar;74(5):2414-9., *And many other. |
| Related products: | - Rat Monoclonal to CD41 (mouse) / Platelet GPIIb - Rat Monoclonal to CD267 / TACI - Rat Monoclonal to CD4 (mouse) |
| Shipping condition: | Room temperature |