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Data sheet of Mouse Monoclonal to CD20
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Human, Non-Human Primates |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to CD20
| Brand: | Exbio |
| Product no.: | 11-638-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to CD20 |
| Antigen: | CD20 |
| Clonality: | Monoclonal |
| Clone: | 2H7 |
| Isotype: | IgG2b |
| Immunogen: | Human tonsillar B cells |
| Format: | purified |
| Specificity: | The mouse monoclonal antibody 2H7 recognizes CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes. _x000D_ HLDA IV; WS Code B201 |
| Categories: | CD Antigens & MHC (Veterinary), Calcium Signaling (Human), CD and Related Antigens (Human) |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *And many other., *Polyak MJ, Ayer LM, Szczepek AJ, Deans JP: A cholesterol-dependent CD20 epitope detected by the FMC7 antibody. Leukemia. 2003 Jul;17(7):1384-9., *Hayden-Ledbetter MS, Cerveny CG, Espling E, Brady WA, Grosmaire LS, Tan P, Bader R, Slater S, Nilsson CA, Barone DS, Simon A, Bradley C, Thompson PA, Wahl AF, Ledbetter JA: CD20-directed small modular immunopharmaceutical, TRU-015, depletes normal and malignant B cells. Clin Cancer Res. 2009 Apr 15;15(8):2739-46., *Diehl SA, Schmidlin H, Nagasawa M, van Haren SD, Kwakkenbos MJ, Yasuda E, Beaumont T, Scheeren FA, Spits H: STAT3-mediated up-regulation of BLIMP1 Is coordinated with BCL6 down-regulation to control human plasma cell differentiation. J Immunol. 2008 Apr 1;180(7):4805-15., *Deans JP, Robbins SM, Polyak MJ, Savage JA: Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment. J Biol Chem. 1998 Jan 2;273(1):344-8., *Deans JP, Kalt L, Ledbetter JA, Schieven GL, Bolen JB, Johnson P: Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20. J Biol Chem. 1995 Sep 22;270(38):22632-8., *Rose AL, Smith BE, Maloney DG: Glucocorticoids and rituximab in vitro: synergistic direct antiproliferative and _x000D_ apoptotic effects. Blood. 2002 Sep 1;100(5):1765-73., *Polyak MJ, Deans JP: Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure. Blood. 2002 May 1;99(9):3256-62., *Chan HT, Hughes D, French RR, Tutt AL, Walshe CA, Teeling JL, Glennie MJ, Cragg MS: CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts. Cancer Res. 2003 Sep 1;63(17):5480-9., *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., *Leukocyte Typing III., McMichael M.J. et al. (Eds.), Oxford University Press (1987); p.611., *Leukocyte Typing Human B Cell and B cell blast-associated Surface Molecules defined with Monoclonal Antibodies, Clerk et al., 1984, pg. 339-346., *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995). |
| Related products: | - Mouse Monoclonal to CD205 - Mouse Monoclonal to CD200 - Mouse Monoclonal to CD203c |
| Shipping condition: | Room temperature |